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Single-chamber differs from dual-chamber bioelectrochemical systems in wastewater treatment and methane recovery under combined exposure to microplastics and antibiotics
Summary
This study compared how single-chamber and dual-chamber bioelectrochemical systems perform when treating wastewater contaminated with both microplastics and antibiotics. Single-chamber systems significantly enhanced methane production by over 21% compared to conventional treatment, while dual-chamber systems struggled due to ammonia buildup. The research found that microplastic and antibiotic contamination dramatically altered microbial communities, highlighting the complex challenges of treating polluted wastewater.
The coexistence of microplastics (MPs) and antibiotics in wastewater poses important threats to microbial ecosystems and methane recovery during anaerobic digestion (AD). This study systematically compares the methanogenic performance and microbial response of single- and dual-chamber bioelectrochemical systems (BES) (0.8 V) exposed to a mixture of MPs (10 mg/L) and antibiotics (1 mg/L). Results demonstrated that single-chamber BES significantly enhanced methanogenesis, achieving a 21.19 % increase in methane production compared to conventional AD, while dual-chamber BES exhibited limited activity due to ammonia inhibition and acetate accumulation. Meanwhile, pollutant exposure dramatically altered the functional enzyme activities and microbial community structure. Metagenomic analysis revealed that methane was primarily produced via the acetoclastic pathway mediated by Methanothrix, with electrical stimulation promoting direct interspecies electron transfer. Pollutant exposure drastically altered microbial communities, reducing Euryarchaeota and enriching fermentative bacteria (e.g., Proteiniphilum). Notably, antibiotic resistance genes (ARGs) increased across all systems, with electrode carriers amplifying ARGs proliferation. However, single-chamber BES showed superior resistance to horizontal gene transfer of ARGs. Key metabolic pathways (e.g., glycolysis, TCA cycle) were markedly inhibited, highlighting the cascading effects of pollutants on microbial energetics. These findings highlight the potential of single-chamber BES for treating co-contaminated wastewater, providing critical insights for optimizing BES configurations.