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Optimized Polyhydroxybutyrate Production by Neobacillus Niacinii GS1 Utilizing Corn Flour, Wheat Bran, and Peptone: A Sustainable Approach
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This study optimized polyhydroxybutyrate production by Neobacillus niacinii GS1, a bacterium isolated from a municipal dump site, using low-cost agricultural residues including corn flour, wheat bran, and peptone as substrates. Response surface methodology identified optimal conditions (37°C, pH 7, 150 rpm) yielding a PHB content of 61.1%, demonstrating a sustainable approach to bioplastic production from waste materials.
Plastic pollution is a pressing environmental challenge, necessitating the development of biodegradable alternatives like polyhydroxybutyrate (PHB). This study focuses on optimizing PHB production by Neobacillus niacinii GS1, a bacterium isolated from a municipal dumping site. By utilizing agricultural residues such as corn flour, wheat bran, and peptone as substrates, we aimed to establish an eco-friendly method for biopolymer production, contributing to sustainable waste management and bioplastic innovation. The bacterium was identified using morphological, biochemical, and molecular techniques. The optimization process involved adjusting variables such as inoculum age, inoculum size, incubation time, agitation rate, incubation temperature, pH of the medium, carbon sources and nitrogen sources. Response surface methodology (RSM) was employed to identify optimal conditions, with the highest PHB yield of 61.1% achieved under specific conditions: 37°C, pH 7, and an agitation rate of 150 rpm. These findings underscore the potential of Neobacillus niacinii GS1 in converting agro-industrial residues into valuable biopolymers, promoting sustainable bioplastic production, and advancing waste valorization efforts through the use of eco-friendly materials.
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Optimized Polyhydroxybutyrate Production by Neobacillus niacini GS1 Utilizing Corn Flour, Wheat Bran, and Peptone: A Sustainable Approach
AI summary Read the abstract
This study optimized polyhydroxybutyrate (PHB) production by Neobacillus niacini GS1 isolated from a municipal dumping site, using corn flour, wheat bran, and peptone as sustainable substrates. Applying response surface methodology, the highest PHB yield of 61.1% was achieved at 37°C, pH 7, and 150 rpm agitation, highlighting the potential of agro-industrial residues for eco-friendly bioplastic production.
Characterization and Process Optimization for Enhanced Production of Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from Bacillus flexus Isolated from Municipal Solid Waste Landfill Site
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Researchers isolated Bacillus flexus bacteria from a municipal landfill and optimized conditions for producing polyhydroxybutyrate (PHB), a biodegradable bioplastic, finding that stress conditions like nitrogen limitation significantly boosted polymer accumulation and yield.
Isolation and Characterisation of Polyhydroxybutyrate (PHB) Producing Bacteria for Sustainable Bioplastic Development
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This paper isolates and characterizes bacterial strains capable of synthesizing polyhydroxybutyrate (PHB), a naturally occurring bioplastic stored as an energy reserve by certain microorganisms. Researchers identified promising bacterial candidates and assessed their PHB yield and production conditions. These findings support ongoing efforts to develop microbially produced bioplastics as biodegradable alternatives to conventional petroleum-based polymers.
Optimization of Polyhydroxybutyrate Production by Bacillus Species Isolated from Dump Site Soil Using Multiple Linear Regression Analysis
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Researchers isolated Bacillus velezensis from landfill soil and optimized conditions to produce polyhydroxybutyrate (PHB), a biodegradable plastic alternative, using sugarcane bagasse as feedstock, achieving a 50% yield and demonstrating full soil biodegradability of the resulting polymer.
Screening, identification and characterisation of Polyhydroxybutyrate producing bacteria from garden soil
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Researchers screened soil bacteria for their ability to produce polyhydroxybutyrate (PHB), a biodegradable biopolymer that can replace petroleum-based plastics. Five promising bacterial isolates were identified and characterized, with production conditions optimized. Scaling up microbial PHB production from inexpensive soil microbes is an important step toward making biodegradable plastic alternatives cost-competitive with conventional plastics.
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